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Standard equipment in the lab includes thirty-four Applied Biosystems 9700 GeneAmp PCR Systems for DNA amplification BioRad iCycler Thermal Cycler for real-time quantitative PCR a BioRad Gel Doc 1000 Video Gel Imaging System for gel documentation a Sorvall Ultra-80 ultracentrifuge for DNA, RNA and protein preparation a Jouan KR422 refrigerated microplate centrifuge for 96-well sample processing, as well as heating/cooling blocks a refrigerated microcentrifuge gel electrophoresis power supplies and chambers and digital programmable multichannel repeat pipettors. The Genomics facilities are outfitted with all the equipment necessary to perform the described research. Several LiCor 6400 open-flow gas exchange systems are available for photosynthesis measurements. Additionally, there is a Carlo-Erba NA1500 analyzer for in-house determination of C and N concentrations of plant tissues, and spectrophotometers for chlorophyll determination. There are two -80☌ deep freezers for sample storage, several rotary evaporators, a freeze drier, and a Speedvac for sample preparation. The lab has a ThermoElectron TEMPUS Fast Gas Chromatograph Time-of-Flight Mass Spectrometer that is configured for both electron impact ionization (EI) and chemical ionization (CI), a Hewlett-Packard HP5980/5972 quadrupole gas chromatograph/mass spectrometer configured in EI mode, an HP1090L high performance liquid chromatograph with a built-in diode array detector, an HP1046A fluorescence detector, and an HP1049A electrochemical detector. The Plant Metabolomics and Physiology facilities have a well-equipped physiology/biochemistry lab that is instrumented for metabolite analyses.
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Facilities and Resources Oak Ridge National LaboratoryĮnvironmental Sciences Division Facilities